Syvn1 Knockout cell line （INS-1）

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location： Home > Products > KO Cell Lines > Syvn1 Knockout cell line （INS-1）

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Syvn1 Knockout cell line （INS-1） Order

Syvn1 Knockout cell line （INS-1）
Catalog#
YKO-H1145
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Syvn1 Knockout cell line （INS-1）
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Syvn1 Knockout cell line (INS-1)
Morphology
Epithelial-like, adherent
Passage ratio
1：2-1：3
Species
Human
Gene Name
Syvn1
Gene ID
361712
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
89%RPMI-1640+10%FBS+1%Sodium pyruvate+ 0.05 mM β-Mercaptoethanol
Target region
Knockout region: Exon 4 (based on transcript Syvn1-201)
Validation: PCR+Sanger Sequencing
Syvn1 Knockout cell line （INS-1） Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Syvn1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the INS-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Syvn1 gene knockout Syvn1 Knockout cell line (INS-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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